Table 2.
Analysis of SCOUTs for p53 mutations
| Sample* | Source | Exons tested | WT/Mut | Nucleotide | Amino acid | Effect | Comment |
|---|---|---|---|---|---|---|---|
| 02N | Normal | E2–E9, E11 | WT | ||||
| 02SC | SCOUT | E2–E11 | WT | Remote to carcinoma | |||
| 02Ca | CA | E2–E4. E6, E8–E11 | Mut | c.1079G>T | G360V | missense | |
| 04N | Normal | E2–E11 | WT | ||||
| 04SC1 | SCOUT | E2–E11 | WT | Remote to carcinoma (Figure 3C) | |||
| 04SC2 | SCOUT | E2–E11 | WT | Remote to carcinoma | |||
| 04SC3 | SCOUT | E2–E11 | WT | Remote to carcinoma | |||
| 04SC4 | SCOUT | E2–E11 | WT | Remote to carcinoma | |||
| 06N | Normal | E2–E5, E7, E9–E11 | WT | ||||
| 06SC | SCOUT | E2–E4, E6, E7, E9–E11 | WT | ||||
| 10N | Normal | E2–E11 | WT | ||||
| 10SC | SCOUT | E2–E11 | Mut | c.659A>C | p.Y220S | missense | Contiguous with carcinoma (Figure 4) |
| 10T | STIC | E2–E11 | Mut | c.659A>C | p.Y220S | missense | |
| 10Ca | Serous carcinoma | E2–E11 | Mut | c.659A>C | p.Y220S | missense | Contiguous with SCOUT (Figure 4) |
Sample numbers are from the original laboratory datasheet. Samples with inadequate DNA amplification were excluded. SCOUT = secretory cell outgrowth; STIC = serous tubal intraepithelial carcinoma; WT = wild type p53 DNA sequence; Mut = p53 mutation detected.