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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Gene Ther. 2010 Apr 29;17(9):1162–1174. doi: 10.1038/gt.2010.56

Figure 1. Schematic diagrams of the plasmid constructs used to produce rAAV.

Figure 1

(A) Map of the pTR-mOP-hGFP plasmid DNA used to make the rAAV2/5-mOP-GFP virus. (B) Map of the pTR-hGRK1-hGFP plasmid DNA used to make the rAAV2/5-hGRK1-GFP virus. (C) Map of the pTR-CBA-hGFP plasmid DNA used to make the rAAV2/5-CBA-GFP virus. TR represents AAV2 inverted terminal repeats; mOP represents a fragment of the proximal mouse opsin promoter; hGRK1 represents a fragment of the human G-protein-coupled receptor protein kinase 1 promoter; CMV ie represents the cytomegalovirus immediate early enhancer; CBA represents the chicken beta-actin promoter; SV40 SD/SA represents the SV40 late viral protein gene 16S/19S splice donor and acceptor signal; hGFP represents the coding sequence of the humanized green fluorescence protein gene; SV40 (poly A) and bGH poly (A) represent polyadenylation signals; HSV-tk represents the thymidine kinase promoter of the herpes simplex virus, and Neo R represents the coding sequence of the neomycin resistance gene.