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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Gene Ther. 2010 Apr 29;17(9):1162–1174. doi: 10.1038/gt.2010.56

Figure 4. Transduction pattern of AAV2/5-mOP-GFP following subretinal delivery to the normal canine retina.

Figure 4

Native GFP fluorescence (green) and GFP immunolabeling (red) images merged with DIC/Nomarski optics; images show the GFP expression with decreasing viral vector titers (A1–4). A1) Retina of E1048-R (3.27 × 1013 vg/ml). A2) Retina of E1048-L (3.27 × 1012 vg/ml). A3) Retina of P1473-R (3.27 × 1011 vg/ml). A4) Retina of P1473-L (3.27 × 1010 vg/ml). Confocal microscopy images of native GFP (green) and cone arrestin (red) immunolabeling in the injected area (B1–3) and non-injected area (B4) of retina E1048-R.

Abbreviations: IS, inner segments; ONL, outer nuclear layer; GFP, green fluorescent protein; IHC, immunohistochemistry; Cone Arr. cone arrestin.

Scale bars: (A1–4) 20 μm; (B1–4) 25 μm.