Native GFP fluorescence (green) and GFP immunolabeling (red) images merged with DIC/Nomarski optics; images show the GFP expression with decreasing viral vector titers (A1–4). A1) Retina of E1050-R (4.79 × 1013 vg/ml). A2) Retina of E1050-L (4.79 × 1012 vg/ml). A3) Retina of P1475-R (4.79 × 1011 vg/ml). A4) Retina of P1475-L (4.79 × 1010 vg/ml). Epifluorescence microscopy images at the border (B1–3) and in the center (B4) of the injected retinal area of E1050-L (4.79 × 1012 vg/ml). Confocal microscopy images of native GFP (green) and cone arrestin (red) immunolabeling in the injected area of retina E1050-L (4.79 × 1012 vg/ml)(C1–3), and retina P1475-R (4.79 × 1011 vg/ml) (C4).
Abbreviations: RPE, retinal pigment epithelium, ONL, outer nuclear layer; GFP, green fluorescent protein; IHC, immunohistochemistry; Cone Arr. cone arrestin.
Scale bars: (A1–4) 20 μm; (B1–4) 40 μm; (C1–4) 10 μm.