Table I.
Summary of dogs injected, promoters used, clinical findings, and sites of GFP expression.
| Promoter | Dog | Sex | Eye | Age at injection (weeks) | Volume injected (μl) | Vector genomes per ml | Injection- termination interval (days) | Clinical findings (days to onset) | GFP expression in rods | Other sites of GFP expression |
|---|---|---|---|---|---|---|---|---|---|---|
| mOP500 | E1048 | M | R | 11.4 | 150 | 3.27 × 1013 | 55 | None | Intense | INL cells, IPL |
| E1048 | M | L | 11.4 | 150a | 3.27 × 1012 | 55 | Focal chorio-retinitis (7) | Intense | - | |
| P1473 | M | R | 19.9 | 150 | 3.27 × 1011 | 69 | Dilated retinal vessels (43) | Moderate | - | |
| P1473 | M | L | 19.9 | 150 | 3.27 × 1010 | 69 | Dilated retinal vessels (43) | Limited | - | |
| hGRK1 | E1053 | M | R | 12 | 150 | 1.51 × 1013 | 48 | Focal chorio-retinitis (41) | Intense | cones (few) |
| E1053 | M | L | 12 | 150b | 1.51 × 1012 | 48 | Focal chorio-retinitis (41) | Intense* | - | |
| E1055 | F | R | 12 | 150 | 1.51 × 1013 | 48 | Focal Retinitis + RD (41) | Intense | cones (few) | |
| E1055 | F | L | 12 | 150a | 1.51 × 1012 | 48 | Focal retinal vasculitis (41) | Intense | - | |
| P1476 | F | R | 19.9 | 150c | 1.51 × 1013 | 69 | Dilated retinal vessels (43) | Intense | - | |
| P1476 | F | L | 19.9 | 150 | 1.51 × 1012 | 69 | Dilated retinal vessels (43) | Intense | INL cells, IPL, GCs | |
| X235 | F | R | 16.6 | 150 | 1.51 × 1011 | 56 | None | Intense | ||
| X235 | F | L | 16.6 | 150 | 1.51 × 1010 | 56 | None | Moderate | ||
| CBA | E1050 | M | R | 11.4 | 150 | 4.79 × 1013 | 26 | Focal retinal vasculitis (20) Panuveitis (23) + RD (26) | Moderate° | RPE, cones |
| E1050 | M | L | 11.4 | 150 | 4.79 × 1012 | 26 | Focal tapetal discoloration (7) | Intense | RPE, cones, IPL, GCs, NFL | |
| P1475 | M | R | 19.9 | 150 | 4.79 × 1011 | 69 | Dilated retinal vessels (43) | Intense | RPE, some cones horizontal cells, Müller cells, GCs, astrocytes, NFL | |
| P1475 | M | L | 19.9 | 150 | 4.79 × 1010 | 69 | Dilated retinal vessels (43) | Limited | RPE |
Abbreviations: M, male; F, female; R, right; L, left; GFP, green fluorescent protein; RD, retinal detachment; IPL, inner plexiform layer; GCs, ganglion cells; RPE, retinal pigment epithelium, NFL, nerve fiber layer.
mOP500: mouse opsin promoter
hGRK1: human G-protein-coupled receptor protein kinase 1 promoter
CBA: CMV immediate early enhancer + chicken β actin proximal promoter
subretinal bleb well formed but with also some sub-RPE injection.
no subretinal bleb formed, injected solution refluxed into the vitreous.
no subretinal bleb formed, injected solution went sub-RPE +reflux into vitreous
, limited to rods at retinotomy site;
limited to rods at the border of retinal detachment.