Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Linearity of CcLpxI activity with time and protein concentration. Panel A. Fraction of 100 μM [β-32P]UDP-2,3-diacylglucosamine converted to the product lipid X at various times under standard assay conditions in the presence of 12.5 nM CcLpxI. Panel B. Fraction of 100 μM [β-32P]UDP-2,3-diacylglucosamine converted to lipid X per minute as a function of enzyme concentration. Panel C. An image of a silica TLC plate of an in vitro CcLpxI reaction run at an enzyme concentration of 40 nM.
