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. Author manuscript; available in PMC: 2011 Aug 10.
Published in final edited form as: Biochemistry. 2010 Aug 10;49(31):6715–6726. doi: 10.1021/bi1008744

Figure 7.

Figure 7

Incorporation of 18O into lipid X during CcLpxI-catalyzed hydrolysis of UDP-2,3-diacylglucosamine in 77% H218O. CcLpxI-catalyzed hydrolysis of UDP-2,3-diacylglucosamine was carried out in the absence or presence of 77% H218O. Panel A. LC/ESI-MS analysis of lipid X generated by CCLpxI in the presence of 100% H216O. Panel B. LC/ESI-MS analysis of lipid X generated by CCLpxI in the presence of H218O/ H216O (77:23, v/v). The insets show LC/ESI-MS analyses of the UMP generated in the reactions.