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. Author manuscript; available in PMC: 2011 Aug 10.
Published in final edited form as: Biochemistry. 2010 Aug 10;49(31):6737–6745. doi: 10.1021/bi100912m

Table 3.

Melting temperature of wild type and R194H mutant of lf- and sfALRa

Construct Tm (°C)
lfALR 86
lfALR R194H 75
sfALR 86
sfALR R194H 78
a

Measured by circular dichroism at 222 nm in 50 mM phosphate buffer, pH 7.5, containing 0.3 mM EDTA (see Experimental Procedures).