Table 3.
Melting temperature of wild type and R194H mutant of lf- and sfALRa
Construct | Tm (°C) |
---|---|
lfALR | 86 |
lfALR R194H | 75 |
sfALR | 86 |
sfALR R194H | 78 |
Measured by circular dichroism at 222 nm in 50 mM phosphate buffer, pH 7.5, containing 0.3 mM EDTA (see Experimental Procedures).