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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Free Radic Biol Med. 2010 Jun 25;49(5):908–917. doi: 10.1016/j.freeradbiomed.2010.06.020

FIG. 1. Initial screening for anti p53-glut antibodies in the serum of 5 immunized rabbits.

FIG. 1

(A) Top panel- Recombinant p53 (rp53) protein was glutathionylated in vitro and the free GSH in the reactions removed by gel-filtration. One μg each of rp53, glutathionylated rp53 or glutathionylated rp53 treated with 10 mM DTT were subjected to non-reducing SDS-PAGE and the resulting blots probed with whole sera from rabbits at 1:1000 dilution. The bottom panel shows equivalent levels of rp53 present in all lanes after reprobing the membrane with monoclonal anti-p53 (DO-1) antibody. (B) Recognition of in vitro cysteinylated rp53 by different rabbit antisera. Cysteinylated rp53 prepared as above was electrophoresed with or without 10 mM DTT exposure and subjected to western blot analysis. Cys, – cysteine.

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