FIG. 3. Detection of glutathionylated p53 protein in human tumor cells using anti p53-glut antibodies.

Induction of glut-p53 protein was determined in HCT116 cells after 0.4 mM H₂O₂ treatment for different times (A), after 20 min incubation with H₂O₂ (0.4 mM), TBH (0.6 mM) and diamide (0.6 mM) (B), with camptothecin (2 μM, 5h) (C), and with camptothecin (2 μM), cisplatin (10 μM), and doxorubicin (5 μM) for 6 and 12 h (C). In the last four lanes of (A) and (B) and last lane of (C) the protein samples were treated with DTT before SDS-PAGE to reverse p53 glutathionylation. C, untreated control; CPT, camptothecin.