FIG. 4. Glutathionylated p53 is selectively degraded in ATP-dependent reactions.

Unmodified rp53 or purified glutathionylated rp53 proteins were incubated in the presence of ATP, Mg⁺⁺, and rabbit reticulocyte lysates as decribed in Methods. Samples were western blotted and probed with DO-1 anti-p53 monoclonal antibodies (lower panel). The band intensities were quantitated and the relative values are shown (upper panel).