Figure 2.

Quantification of fiber distance distribution with in-house software: (A) representative raw image data shown as z-projection of the Col1 fibers (red) in a hypoxic (green) region; (B) representative example of software detection of hypoxic cells (green in raw image (A)) independent of fluorescence intensity and threshold of raw images; preprocessing using the filter (C) to identify fiber-like objects (red) among various non-fiber-like objects in the image to produce (D) the corresponding Col1 fiber image; (E) computing the distances three-dimensionally to the surrounding fibers from each voxel to determine the nearest distance to a fiber. The fiber volume is computed as an additional parameter.