Figure 3.

MAb A4 recognizes protocadherin β₁₃ on melanoma cells. (A) TL of B16F10-Nex2 was examined for reactivity with mAb A4. TL, a band at 87 kDa was seen on SDS-PAGE; WB, a protein of identical molecular mass was recognized by mAb A4 in Western blot analysis; IP, the 87-kDa component was immunoprecipitated by mAb. (B) A mAb A4-immunoprecipitated sample was analyzed by LC-MS/MS, and the peptide shown underlined in bold italic and below was fully identified, being a characteristic of protocadherin β₁₃ (complete sequence shown, 796 aa, calculated mol. wt. = 87,458).