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. 2010 Aug 1;3(4):204–217. doi: 10.1593/tlo.09316

Figure 6.

Figure 6

MAb A4 VH CDR3 (H3) peptide is the only inhibitory CDR of mAb A4 (A). A4 H3 peptide induces DNA degradation in B16F10- Nex2 cells (B). STD, 1 Kb Plus DNA Ladder (Invitrogen). (C) Cyclic-extended and linear mAb A4 H3 peptides were tested for competition with mAb A4 in CL-ELISA. Peptides were incubated with tumor cells previously to addition of mAb A4 as described in Materials and Methods. (D) In vitro titration of cytotoxic effects of cyclic and linear mAb A4 H3 in B16F10-Nex2 cells. Black bar, untreated cells (*P < .001). (E) Addition of cyclic-extended mAb A4 H3 peptide (50 µg) abrogates mAb A4 binding to B16F10-Nex2 cells (blue peak). Negative control, cells incubated with anti-IgG FITC alone. Positive control, mAb A4 alone (red peak).