Table 2.

qPCR Analysis of the Effect of Forced Expression of CD24 on mRNA Levels of Several Key Genes Functionally Involved in Oncogenicity and Neoplastic Progression of EC Cells^*.

Functional Gene Grouping	Gene	Fold Change†	P
Cell cycle control and DNA damage repair	CCND1	3.92	1.36e-06
	ATM	2.14	2.71e-02
	BRCA1	2.26	6.49e-06
	Cyclin E1	1.90	2.19e-05
	CDC25A	3.09	2.10e-03
	CDK2	3.13	2.94e-02
	CDKN1A	1.75	1.86e-02
	CDKN2A	1.53	2.81e-02
	CHEK2	1.72	6.10e-04
	E2F1	2.48	4.36e-04
	CDKN1B	-1.31	9.19e-04
	RB1	2.03	2.81e-05
Apoptosis and cell senescence	S100A4	1.79	4.46e-05
	TP53	-1.84	1.03e-04
	APAF1	2.20	3.16e-04
	BAD	-3.20	5.91e-03
	BAX	2.19	1.28e-04
	BCL2	3.64	2.35e-05
	BCL2L1	1.86	6.11e-03
	CASP7	2.11	1.44e-06
	HTATIP2	-7.28	1.43e-02
	TERT	2.89	3.39e-02
	TNFRSF1A	2.91	1.98e-03
	TNFRSF10B	2.06	2.57e-04
Signal transduction molecules and transcription factors	AKT1	2.27	6.11e-06
	ERBB2	3.61	4.86e-03
	ETS2	2.16	2.80e-05
	FOS	1.86	2.54e-02
	JUN	2.03	4.43e-04
	MAP2K1	2.05	1.19e-02
	MYC	2.19	8.43e-03
	NFKB1	2.34	1.16e-04
	NFKBIA	2.04	1.60e-04
	PIK3R1	2.77	6.79e-03
	RAF1	2.75	5.59e-03
	SNCG	1.81	1.24e-05
	TWIST1	2.46	2.31e-02
Invasion and metastasis	MET	2.06	7.36e-06
	MTA1	2.38	2.50e-02
	MTA2	2.28	4.26e-03
	NME1	1.69	4.56e-02
	PLAU	-2.83	8.21e-04
	PLAUR	1.85	2.41e-03
	SERPINB5	2.08	1.59e-02
	Cell remodeling	VIM	35.40 1.57e-02
	OCLN	-2.85	4.23e-04
	FN1	1.93	2.17e-02

Positive and negative fold changes indicate an increase or a decrease in mRNA levels respectively. A list of the genes analyzed by real-time PCR and the primer sequences used are previously described [2].

^*Results are presented as a fold change in mRNA levels in RL95-2-CD24 cells relative to RL95-2-vector cells.

^†Fold change values are representative of three independent experiments of mRNA samples.