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. Author manuscript; available in PMC: 2010 Aug 4.
Published in final edited form as: Nat Protoc. 2010 Mar 11;5(4):636–646. doi: 10.1038/nprot.2010.3

Figure 6.

Figure 6

TLC analysis of enzymatic synthesis of N-acetylglucosamine 1-phosphate (GlcNAc-1-P) and N-acetylgalactosamine 1-phosphate (GalNAc-1-P) using N-acetylhexosamine 1-kinase (NahK). The enzymatic reactions were carried out for 19 h. The developing solvent used was n-butanol:acetic acid:water = 2:1:1 (v/v/v). The plate was stained with p-anisaldehyde sugar stain. Lane: 1, N-acetylglucosamine (GlcNAc); 2, GlcNAc-1-P authentic sample; 3, reaction mixture of GlcNAc using NahK; 4, N-acetylgalactosamine (GalNAc); and 5, reaction mixture of GalNAc using NahK.