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. Author manuscript; available in PMC: 2010 Aug 4.
Published in final edited form as: Nat Protoc. 2010 Mar 11;5(4):636–646. doi: 10.1038/nprot.2010.3

Figure 8.

Figure 8

TLC analysis of enzymatic synthesis of GDP–fucose. The developing solvent used was n-butanol:acetic acid:water = 2:1:1 (v/v/v). The plate was stained with p-anisaldehyde sugar stain. Lane: 1, l-fucose; 2, ATP; 3, reaction mixture without l-fucose pyrophosphorylase (FKP); 4, reaction mixture without GTP; and 5, alkaline phosphatase-treated reaction mixture of GDP-fucose synthesis.