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A, Western blot analysis of SERCA2, phospholamban (PLB) and calsequestrin expression at different ages. Upper panel: representative original recording. Lower panel: mean values of protein content (n= 8 for each age). B, ratio of α-MHC to β-MHC mRNA expression measured by real-time quantitative RT-PCR. N= 8 animals for each age. *P < 0.05, **P < 0.01, ***P < 0.001 versus adult age (63 days).
