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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Curr Protoc Cytom. 2010 Jul;CHAPTER:Unit–9.34. doi: 10.1002/0471142956.cy0934s53

Figure 4. Dump Channel Analysis.

Figure 4

This figure shows the effective use of a “DUMP” channel, which uses negative gating strategy. In this strategy, ViViD is combined with anti-CD20 Pacific Blue (PB) and anti-CD14 PB to exclude dead cells, B-cells, and monocytes (respectively) using a single detector. After compensation, five populations can be discriminated based on the ViViD and MAB-conjugates (Figure 4A), as follows: live CD3+ T-cells (1), dead CD3+ T-cells (2), monocytes (3), B-cells (4), and live CD3- CD14- CD20- cells (5). Figures 4B-F shows the overlay of the different populations (1-5) over the lymphocyte gate containing the CD3+ T-cells (1). Each population shows the degree of contamination within the traditional lymphocyte gate, dead cells (C), monocytes (D), B-cells (E) and live CD3-14-20- cells (F).