Figure 8. CD3+ T cells that are dimly stained with amine reactive dyes should be included in cell analysis.

Figure 8 shows the proliferative capacity of three CD3+ ViViD stained populations, ViViV-Low, ViViD-Mid and ViViD-High after staining with CFSE, ViViD and anti-CD3-Cy7APC. The 2^nd row shows the sort purity results for each of the sorted cell populations as illustrated in the histogram in the 1^st row. The 3^rd and 4^th rows show results of post incubation for five days in the presence of SEB (Streptococcus Enterotoxin B) followed by staining with OrViD (amine reactive dye measured in G610 detector) and anti-CD3-APC. These additional stains were added to measure dead cells as a result of a 5-day incubation and the total number of CD3+ T cells. In the 3^rd row three populations can be described, live cells (A, ViViD-OrViD-), dead cells as a result of incubation (B, ViViD-OrViD+) and dead cell from the original sort (C, ViViD+OrViD+). In both the ViViD-Low and ViViD-Mid cell sorts, live cells were observed and these cells were also proliferating (4^th row). The dead cells from the ViViD-High cell sort show no cell proliferation (4^th row).