| Dilution | Dye wt. (ug) |
DMSO (ul) |
Stock Conc. (ug/ml) |
Working Conc. (ug/ml) |
Final Conc. (ug/ml) |
|---|---|---|---|---|---|
| 1 | 25 | 50 | 500 | 12.50 | 0.625 |
| 2 | 25 | 100 | 250 | 6.25 | 0.313 |
| 3 | 25 | 200 | 125 | 3.12 | 0.156 |
| 4 | 25 | 400 | 62.5 | 1.56 | 0.078 |
| 5 | 25 | 800 | 31.25 | 0.78 | 0.039 |
| 6 | 25 | 1600 | 15.62 | 0.39 | 0.020 |
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