Table 15.1.2.
Summary of General Parameters for Various EAE Adoptive Transfer Modelsa
Mouse strain | Antigen specificity | Donor immunization period (days) | In vitro antigen concentration (mg/ml) | In vitro IL-12 (ng/ml) | In vitro culture Time (hr) | Number of blasts transferred (×106) | Disease type | Disease severity |
---|---|---|---|---|---|---|---|---|
SJL | PLP | 7-14 | 50-100 | — | 72-96 | 5-10 | Relapsing-remitting | Severe |
PLP139-151 | 7-14 | 20 | — | 72-96 | 1-5 | Relapsing-remitting | Severe | |
MBP | 7-14 | 50-100 | — | 72-96 | 40-60 | Relapsing-remitting | Moderate | |
MBP84-104 | 7-14 | 50 | — | 72-96 | 10-20 | Relapsing-remitting | Moderate | |
C57BL/6 | MBP | 10-14 | 50-100 | — | 72-96 | 50 | Monophasic/chronic | Mild |
MBP84-104 | 10-14 | 50 | — | 72-96 | 50 | Monophasic/chronic | Mild | |
MOG | 10-14 | 50 | 25 | 72-96 | 20 | Chronic | Moderate | |
MOG35-55 | 10-14 | 10 | 25 | 72-96 | 20 | Chronic | Moderate | |
B10.PLb | MBPAc1-11 | — | 50 | 10 | 72 | 1 | Monophasic | Moderate |
B10.S | MBP | 10-11 | 25 | 20 | 96 | 35 | Monophasic | Moderate |
MBP87-106 | 10-11 | 50 | 20 | 96 | 35 | Monophasic | Moderate |
These parameters have been optimized in different laboratories as previously described. SJL protocol parameters are from Pettinelli and McFarlin (1981), Pettinelli et al. (1982), Miller et al. (1991), McRae et al. (1992), Fritz and Zhao (1994), Segal et al. (1994), Skundric et al. (1993, 1994), and Tuohy and Thomas (1995). C57BL/6 protocol parameters are from Mendel et al. (1995), Shaw et al. (1995), Clark et al. (1997), Segal et al. (1998), Mendel and Shevach (2002), and Tompkins et al. (2002). B10.PL protocol parameters are from Walker and Mannie (2002). B10.S protocol parameters are from Segal and Shevach (1996).
Note that the B10.PL system employs T cell receptor transgenic donors which do not require in vivo priming, only in vitro culture with MBPAc1-11 peptide and rIL-12.