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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Mol Microbiol. 2010 May 24;77(1):56–73. doi: 10.1111/j.1365-2958.2010.07222.x

Figure 2.

Figure 2

DipM is required for efficient cell division and normal cell morphology. A. Morphology of cells from a strain bearing PxylX-dipM on a high copy number plasmid (EG301) grown in PYE with 0.2% glucose or 0.3% xylose for 17 h. Arrows indicate rounded cells. B. Genomic context of dipM and strategy for generating deletion and depletion strains. C. Spot dilutions of the indicated strains after 3 days of growth at 30°C. Three independent ΔdipM isolates are shown. D. Morphology of ΔdipM cells (EG590). Smooth filaments (asterisk); shorter, wide cells (arrow); and cells with envelope roughening (hatched arrow) were observed. E. Cells from DipM depletion strain EG353 grown for the indicated amount of time in PYE without xylose inducer. F. Immunoblots using anti-DipM antibodies or anti-HU2 control antibodies of CB15N (WT), ΔdipM (Δ), and EG353 cells at 0, 3, 6, 9, 15, and 21 h of growth without xylose. Bars = 2 µm.