Table 1.

siRNA sequences, target sites in human, mouse, and rat mRNA of BACE1 and silencing activity of used siRNA- and shRNA-coded plasmids (presented as % of BACE1 gene expression).

		Target gene (nt numbers)	% of BACE-GFP expression in DFA by siRNA (5/1/0.1 nM)*	% of BACE-GFP expression in DFA by shRNA in plasmid∗#	% of BACE1 expression in SH-SY5Y (human) cells by siRNA (RT-PCR/western blot)∗&	% of BACE1 expression in HEK293 (human)/M15 mice cells by shRNA in pcPURhU6@ (RT-PCR)
	Sequence:
No	5′-sense strand-3′
	3′-antisense strand-5′
si-0	5′-AAUCAGAUUGAACCUUCAUTT-3′	non-silencing control siRNA	100	100	86	100
	3′-TTUUAGUCUAACUUGGAAGUA-5′
si-1	5′-UACAGGCAGCAGUAACUUUTT-3′	h (733–753)m (-)r (-)	27/31/58	40 (pSilencer)	nd	nd
	3′-TTAUGUCCGUCGUCAUUGAAA-5′
si-2	5′-AGACGCUCAACAUCCUGGUTT-3′	h (710–730) m (-)r (-)	6/22/76	nd	61/60	nd
	3′-TTUCUGCGAGUUGUAGGACCA-5′
si-3	5′-GAAUCAGACAAGUUCUUCAUC-3′	h (946–966)m (-)r (-)	0.0/1.1/16	5 (pSilencer)	55/61	nd
	3′-GACUUAGUCUGUUCAAGAAGU-5′
si-4	5′-AAUCAGACAAGUUCUUCAUTT-3′	h (947-967)m (-)r (-)	0.0/1.7/15	11 (pSilencer)	34/61	nd
	3′- TTUUAGUCUGUUCAAGAAGUA-5′
si-5a	5′-AUCAGACAAGUUCUUCAUCAA-3′	h (948–968) m (-)r (-)	28/35/60	12 (pcPURhU6)	nd	32/92
	3′-GAUAGUCUGUUCAAGAAGUAG-5′
si-5b	5′-AUCGGACAAGUUCUUCAUCAA-3′	h (-)m (920–940)r (920–940)	nd	nd	nd	52/30
	3′-GAUAGCCUGUUCAAGAAGUAG-5′
si-6	5′-GACUGUGGCUACAACAUUCCA-3′	h (1777–1797)m (1751–1771)r (1751–1771)	1.3/8/45	20 (pcPURhU6)	nd	51/71
	3′-UUCUGACACCGAUGUUGUAAG-5′

*: GFP/RFP relative fluorescence of the control, HeLa cells transfected with control nonsilencing siRNA (si-0) or pSilencer-Neg plasmid/Lipofectamine 2000 were taken as a reference nonsilenced value of 100%;

^#: plasmid DNA 30 ng/well (96-well plate);

^&: 100 nM siRNA;

^@: plasmid DNA 2 μg/well (6-well plate);

Nd: not determined.