Table 2.
List of genes selected for expression level evaluation in HCN A94 cells transfected with BACE1-specific si-5b and si-6, coded in lentivirus vector.
| No | Protein | Name/Function | % of silencing with Lv-si-8 | % of silencing with Lv-si-9 |
|---|---|---|---|---|
| 1 | BACE1 | Beta-site APP cleaving enzyme 1 | >70 | >75 |
| 2 | BACE2 | Beta-site APP cleaving enzyme 2 | — | — |
| 3 | SCG10 | Superior cervical ganglion-10 | 70 | 70 |
| 4 | NeuroD1 | Neurogenic differentiation 1 | — | — |
| 5 | GluR2 | Glutamate receptor 2 | — | — |
| 6 | CREB | cAMP responsive element binding protein 1 | — | — |
| 7 | MeCP2 | Methyl CpG binding protein 2 | — | — |
| 8 | Synapsin 1 | Protein involved in the regulation of neurotransmitter release at synapses | — | — |
| 9 | PKR | Double stranded RNA-dependent protein kinase | — | — |
| 10 | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | — | — |
| 11 | Sox-2 | SRY (sex determining region Y)-box 2 | — | — |
| 12 | NRSF/REST | Neural restrictive silencing factor | — | — |
| 13 | NaChII | Sodium channel, voltage-gated, type II | — | — |
| 14 | βIII-Tubulin | Neuro-specific tubulin, microtubules component | — | — |
| 15 | Calbidin | Vitamin D-dependent calcium-binding protein | — | — |
| 16 | BDNF | Brain-derived neurotrophic factor | — | — |
| 17 | GFAP | Glial fibrillary acidic protein, protein specific for astrocytes in CNS | — | — |
| 18 | APP | Amyloid Precursor Protein | — | — |
| 19 | Neurogenin 1–3 | Neurogenins, family of the transcription factors involved in the neuronal differentiation | — | — |