Fig. 5.

Quantitative PCR analyses of candidate target genes. Quantitative ChIP–PCR analyses were conducted to validate the potential target genes of nuclear RON. After serum starvation for the indicated time periods, TSGH8301 cells were harvested and ChIP assays were performed with antibodies directed against RON (A) or EGFR (B). The immunoprecipitation DNA corresponding to the interested targets was measured by quantitative PCR. Quantitation of specific RON targeting was determined as a percent of input DNA and each error bar represents standard deviation calculated from triplicates. (C) Quantitative real-time–PCR analysis was performed to investigate the expression level of candidate target genes. Each error bar represents standard deviation calculated from triplicates. Amplified samples without reverse transcription were used as negative controls.