Figure 2.

In vitro and in vivo function of NTA₃-PTD. (A) H1299 cells were treated with NTA₃-PTD + Ni plus 6xHis-rhodamine peptide or 6xHis-rhodamine peptide alone for 30 min, washed, and assayed by flow cytometry. (B) H199 cells were treated with NTA₃-PTD plus 6xHis-β-Gal, 6xHis-β-Gal alone, or positive control TAT-β-Gal fusion protein for 2 h, washed, and incubated with C₁₂FDG substrate for 45 min, followed by flow cytometry. (C) CHO-ZEG cells were treated with NTA₃-PTD plus 6xHis-Cre or 6xHis-Cre alone for 30 min, washed, trypsinized, and replated. After 24 h, cells were assayed for GFP expression via flow cytometry. (D) ROSA-26R-LoxP-STOP-LoxP-luciferase mice were subcutaneously injected with 50 μL of 20 μM NTA₃-PTD plus 6xHis-Cre or 6xHis-Cre alone and then assayed for luciferase expression at 4 days.