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. 2010 Mar 15;2010:305192. doi: 10.1155/2010/305192

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Copyright © 2010 Mineko Fujimiya et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Localization of ghrelin, des-acyl ghrelin and obestatin in the rat stomach. (a) Immunofluorescence double staining for acyl ghrelin- (red) and des-acyl ghrelin-positive (green) reaction in the antral mucosa of rat stomach. Acyl ghrelin-positive reaction and des-acyl ghrelin-positive reaction are colocalized in closed-type cells (arrows), whereas des-acyl ghrelin-positive reaction is localized in open-type cells (arrowheads). (b) Immunofluorescence triple staining for des-acyl ghrelin (green), acyl ghrelin (red) and obestatin (blue) in the antral mucosa of rat stomach. Three peptides are colocalized in the closed-type cells (arrows). Bars = 10 μm.