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cAMP binding to MSMEG_5458. A, displacement of [3H]cAMP from MSMEG_5458 (∼50 nm) by unlabeled cyclic nucleotides (10 μm). Values represent the mean ± S.E.; n = 3. B, displacement of [3H]cAMP (∼50 nm) from MSMEG_5458 with increasing concentrations of the indicated ligands; n = 3. Values represent the mean ± S.E. C, binding of [3H]cAMP (∼50 nm) to MSMEG_5458R95K. Values shown represent the mean ± S.E. of duplicate determinations in experiments repeated thrice.
