FIGURE 7.

Biochemical properties of Rv0998. A, displacement of [³H]cAMP (∼50 nm) from Rv0998 (5 μg) with increasing concentrations of unlabeled cAMP. Values represent the mean ± S.E. with n = 3. B, acetylation of USP by Rv0998 was monitored by Western blot analysis with acetyl-lysine antibodies performed in the presence or absence of cAMP (1 mm, upper panel) followed by staining of the blot with Coomassie Brilliant Blue (lower panel). C, acetyltransferase activity of Rv0998 using the coupled assay, in the absence or presence of 1 mm cAMP. Data are representative of assays performed thrice.