FIGURE 2.

Expression and subcellular localization of tagged Yat1 and Yat2. A, immunoblot analysis with α-HA antibody of the wild type and cat2 null strain expressing pMAL2–3×HA-Yat1 and pMAL2–3×HA-Yat2 constructs. Strains were grown on rich oleate medium (YPO) or on YPO + 0.5% maltose (YPO/M) to induce expression from the MAL2 promoter. B, immunoblot analysis of a subcellular fractionation experiment of the 3×HA-Yat1 and 3×HA-Yat2 strains grown on YPO or YPO/M. The total homogenate (H) was fractionated into an organellar pellet fraction (P) and a cytosolic supernatant fraction (S). Thiol, thiolase (peroxisomal marker protein). Cit1, mitochondrial marker protein; Hxk1, cytosolic marker protein. All experiments were performed at least twice; representative experiments are shown.