FIGURE 4.

Growth phenotypes of the different carnitine acetyltransferase mutant strains. A, serial dilutions (1:10) of the indicated strains were spotted on minimal plates with glucose, oleate, lactate, citrate, acetate, or ethanol as a carbon source and incubated for 3–5 days at 28 °C before pictures were taken. B, growth curve of the wild type and mitCAT2 strains on minimal oleic acid medium without or with 10 μm l-carnitine. C, growth curve of the wild type and mitCAT2 strain on minimal acetate medium without or with 10 μm l-carnitine. D, growth curve of the wild type and mitCAT2 strain on minimal ethanol medium without or with 10 μm l-carnitine. E, growth curves of the wild type and c2/y2/y1Δ/Δ + CAT2 stains on minimal oleate medium without or with 10 μm l-carnitine. All experiments were performed at least twice; representative experiments are shown.