FIGURE 6.

ATP hydrolysis by FMDV 2C is coordinated. a, various ratios of wild type 2C(34–318) (WT) and 2C(34–318) Walker A mutant, K116A (MUT), were mixed before addition to an ATPase assay. b, various ratios of wild type 2C(61–318) and 2C(61–318) Walker A mutant, K116A, were mixed before addition to an ATPase assay. Asynchronous ATP hydrolysis would be expected to decrease linearly as the amount of inactive enzyme is increased (dotted line in both plots). Data are averages of three experiments ± S.E. (error bars).