FIGURE 7.
Nuclear-inducible DUSP mRNA expression after brief or sustained GnRH treatment. Cells were plated in 6-well plates, serum-starved overnight, then treated for 4 h with 10−7 m GnRH either continuously or with GnRH or control for 5 min. Total RNA was isolated and analyzed for relative levels of DUSP1, -2, -4, or -5 mRNA using quantitative PCR as described under “Experimental Procedures.” Data shown are normalized values (mean ± S.E.) obtained from three independent experiments, each with duplicate readings. Significant differences are indicated, comparing untreated control versus treated using one-way ANOVA and Bonferroni's post-hoc test; *, p < 0.05; **, p < 0.01; ***, p < 0.001.