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. 2010 Jun 3;285(32):24529–24537. doi: 10.1074/jbc.M110.127779

FIGURE 3.

FIGURE 3.

Titration of the fluorescence emission spectra of AKR1D1 with increasing [NADPH]. A, emission spectrum of recombinant AKR1D1 excited at 295 nm following the addition of increasing concentrations of NADPH from 0 to 4 μm. A decrease in emission is seen at 340 nm, and an increase in emission is seen at 460 nm. B, plot of ΔFFmax versus [NADPH], wild type (■), and P133R (▴).