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. 2010 Jun 3;285(32):24529–24537. doi: 10.1074/jbc.M110.127779

FIGURE 5.

FIGURE 5.

Detection of AKR1D1 and AKR1D1 mutant mRNA following transient transfection in HEK 293 cells. HEK 293 cells were transfected with wild type (+Wt) and mutant AKR1D1 (+L106F, +P133R, +P198L, and +R261C), and expression was monitored with primer pairs specifically amplifying either endogenous or vector-expressed (exogenous) AKR1D1. The liver cell line HepG2 expressed AKR1D1 endogenously and served as a positive control for this primer pair. 1D1-pcDNA3.1 is the purified plasmid of the wild type construct transfected into HEK 293 cells and serves as positive control for detection of the exogenous AKR1D1.