FIGURE 4.

Intramitochondrial localization of CYP2E1 in stable expression cells. Mitochondria from WT (left hand panel) and Mt++ cells (right panel) were treated with digitonin and or trypsin as described under “Experimental Procedures.” In the indicated experiments, mitochondria were treated with 0.2% Triton X-100 (v/v) before treatment with digitonin or trypsin. Proteins (50 μg each) were resolved on 12% SDS-PAGE and subjected to immunoblot analysis with CYP2E1 antibody. The blots were co-developed with antibodies to the 70-kDa subunit of complex II and Tim23. Blots from identically run companion blots were used for developing with antibodies to CcO I subunit and TOM20.