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Iron-dependent PTP inhibition is specific to Fe-cit. Mϕ PTP activity was determined following incubation of (A) 10 μg of B10R total protein lysates (B) or GST-purified recombinant PTPs for 10 min at RT with Fe-cit, FeSO4 or FeCl3 to a final concentration of 500 μm. PTP activity was evaluated by pNPP hydrolysis and absorbance reading at 405 nm. Statistically significant differences (**) were considered when p < 0.01.
