FIGURE 4.
A DHHC15 protein containing the DHHC-CR domain from DHHC3 is inactive against SNAP23. A, schematic highlighting the make-up of the DHHC15/3 chimeric protein is shown. B, HEK293T cells were transfected with EGFP-SNAP23 (or GFP) together with pEFBOS-HA (control), HA-DHHC3, HA-DHHC15, or HA-DHHC15/3. Cells were labeled with [3H]palmitic acid acid, lysed, and resolved by SDS-PAGE. The top panel shows [3H]palmitate incorporation, the middle panel shows expression levels of EGFP-SNAP23, and the bottom panel shows DHHC protein expression. Molecular weight markers are shown on the left. C, HA-DHHC15/3 was expressed in HEK293T cells, labeled with Alexa Fluor 488-conjugated anti-HA, and visualized using confocal microscopy. The scale bar represents 5 μm.