FIGURE 5.

HAS2 effects on IL-1β-induced monocyte adhesion. A, relative quantification of the transcript coding for HAS2 in HUVECs silenced for HAS2 and treated with IL-1β (black bars). Cells were left untreated (white bars) or transfected with 50 μm of scramble siRNA (si-scramble) or with 50 μm of HAS2 siRNA (si-HAS2). After 24 h of incubation, 5 μg/ml of IL-1β was added, and, after 24 h, RNA was extracted for quantitative RT-PCR experiments. The lowest HAS2 mRNA level in three different untreated samples was set at 1, and the S.E. is shown on each bar. Results are expressed as mean ± S.E. *, p < 0.01 untreated versus treated samples. B, the adhesion assay was done on HUVECs silenced for HAS2 and treated with IL-1β. 24 h after siRNA transfections and subsequently after 24-h treatment with 5 μg/ml IL-1β, fluorescent monocytes were added, washed, and counted. Results are expressed as the number of adherent U937 per field and represented as mean ± S.E. of six independent fields. *, p < 0.01; untreated versus the treated samples.