FIGURE 7.

Activation of β-catenin promotes TCF binding to the proximal TBE in PAI-1 promoter. A, partial sequence of PAI-1 gene promoter region. Bold letters indicate the putative TBE. P1 and P2 indicate the primer pair encompassing the proximal TBE, whereas P3 and P4 indicate the prime pair for the distal TBE. B and C, ChIP assay revealed that activation of β-catenin (either by TGF-β1 or by overexpressing stabilized β-catenin) promoted TCF binding to the proximal TBE but not distal TBE. Representative ChIP assay (B) and quantitative ChIP data (C) are presented. *, p < 0.05 versus controls; **, p < 0.01 versus controls; †, p < 0.05 versus TGF-β1 or pDel-β-cat alone (n = 3).