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. 2010 Jun 4;285(32):24676–24685. doi: 10.1074/jbc.M110.106278

FIGURE 4.

FIGURE 4.

Expression of WT and mutant V-ATPase A subunit in HEK-293T cells modulates subcellular localization of the A subunit and extracellular pH. HEK-293 cells were transfected with either vector alone (A) or FLAG-tagged WT (B), S175A (C), or S175D (D) mutant A subunit 1 day prior to immunofluorescence staining for expression of FLAG (green), concanavalin A coupled to CY3 as a membrane marker (red), and TO-PRO-3 nuclear stain (blue). Scale bar = 15 μm. E, mean (±S.E.) extracellular pH (pHo) of the culture medium from HEK-293T cells transfected with different plasmids after 28–31 h incubation (*, p < 0.0001 relative to vector alone; #, p < 0.0001 relative to WT; n = 15–18).