FIGURE 3.

The SP and CRE binding sites are required for ITGB8 promoter activity. A, mutant ITGB8 reporter constructs and activities in H1264, U373, and HeLa cell lines. All constructs are drawn to scale with mutated sites indicated by dashed lines. Fold-induction is as defined as described in the legend to Fig. 2. B, mutant ITGB8 reporter assays in primary HBEC, adult lung fibroblasts, and fetal astrocytes (±S.E.). Baseline is indicated by a line. The empty bar is baseline, pSEAP basic, whereas filled bars represent relative activity of mutant reporter constructs indicated at the left. * = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001.