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. 2010 Jun 6;285(32):24717–24728. doi: 10.1074/jbc.M109.088229

FIGURE 5.

FIGURE 5.

Embryonic phenotypes of pst-1 and pst-2. A, percentage of embryonic mutant phenotypes in pst-1(tm3364), pst-2(tm3316), and double pst-1(tm3364);pst-2(tm3316) mutants. N.S., not significant. ***, p < 0.001. B and C, EMS cell division axis is affected in pst-1(tm3364) embryos. In wild-type embryos at the 6-cell stage, EMS cells divide along the anterior/posterior axis (bidirectional arrow (B)). In pst-1(tm3364) mutant embryos at the 6-cell stage, the axis of EMS division is skewed (bidirectional arrow (C)). D and F, DIC images of wild-type (D) and pst-1(tm3364) (F) embryos at the ventral cleft enclosure stage. The dashed lines indicate the edges of an unenclosed ventral surface. E–G″, time lapse analysis of wild-type (E and E′) and pst-1(tm3364) (G and G″) embryos during the elongation stage. Arrows indicate swollen body morphology. Scale bar = 10 μm.