FIGURE 6.

Expression patterns of pst-1 and pst-2 reporter constructs in wild-type animals. Transgene reporter analyses of pst-1 (A–D) and pst-2 (E and F). DIC (A–F) and fluorescent (A′–F′) images are shown. GFP was visualized directly under a fluorescence microscope except in B′ and C′, which were sliced samples immunostained with an anti-GFP antibody. A and A′, expression pattern of P_pst-1bc::egfp in a folded embryo. EGFP is dominantly expressed in seam cells. B, B′, C, and C′, expression pattern of pst-1ab(fl)::egfp in an embryo (B) and the mid-ventral region (C) of a mid-L4 hermaphrodite. EGFP is dominantly expressed in seam cells. EGFP is expressed in seam cells (SCM) and the hypodermis (indicated with dashed lines) but not in the vulva (C′). D and D′, expression pattern of P_pst-1a::egfp in a mid-L4 larva. EGFP is expressed in almost all cells. E and E′, expression pattern of P_pst-2a::egfp in a young adult animal. EGFP is expressed in the intestine and pharyngeal gland cells. GFP expression in pharyngeal gland cells is weaker than in the intestine in this animal. F and F′, expression pattern of pst-2(fl)::egfp in the head region of a young adult animal. The EGFP signal in the pharyngeal gland cells is indicated with an arrowhead. Scale bars = 10 μm (A–C) and 100 μm (E–F).