TABLE 2.
Disaccharide composition of HS from C. elegans wild-type (N2) and mutant (pst-1 and pst-2) strains
| Disaccharidea | N2 | pst-1b | pst-2c |
|---|---|---|---|
| pmol/mg HS (%) | |||
| ΔHexAα1–4GlcNAcc | 23.6 ± 0.3 (47) | 35.3 ± 0.8 (69) | 22.6 ± 2.7 (71) |
| ΔHexAα1–4GlcNAc(6S) | 4.8 ± 1.2 (10) | 5.3 ± 3.0 (10) | 1.5 ± 0.3 (5) |
| ΔHexAα1–4Glc(NS) | 12.9 ± 1.7 (26) | 8.2 ± 3.2 (17) | 4.9 ± 2.3 (15) |
| ΔHexA(2S)α1–4Glc(NS) | 7.2 ± 1.9 (14) | 1.6 ± 0.5 (3) | 1.7 ± 1.0 (5) |
| ΔHexA(2S)α1–4Glc(NS,6S) | 1.6 ± 0.5 (3) | 0.7 ± 0.1 (1) | 1.0 ± 0.4 (3) |
| Total disaccharide | 50.1 ± 3.5 | 51.1 ± 5.9 | 31.7 ± 3.6 |
| Sulfation degreed | 0.74 | 0.37 | 0.40 |
a ΔHexAα, Glc, and GlcNAc represent unsaturated hexuronic acid, glucosamine, and N-acetylglucosamine, respectively.
b Liquid culture samples containing pst-1(tm3364)/nT1[qIs51] heterozygotes and pst-1(tm3364)/pst-1(tm3364) homozygotes were used.
c Liquid culture samples containing only pst-2(tm3316) homozygous animals were used.
d Sulfation degree is expressed as the average number of sulfate groups/disaccharide unit.