TABLE 4.
Either neuroectodermal or epidermal expression of PST-1 was necessary and sufficient for embryogenesis
| Promoter | Target tissue | No. of plates with proliferating progeny/total test platesa | Rescue activityb |
|---|---|---|---|
| 10 larvae/plate | |||
| None | 0/2 | − | |
| pst-1bc | Hypodermis, seam cells, amphid sheath | 2/2, 2/2c | ++ |
| dpy-7 | Hypodermis, seam cells | 3/5, 3/3c | + |
| unc-119 | Neuroectoderm, differentiated neurons | 2/2, 2/2c | ++ |
| rgef-1 | Differentiated neurons | 0/3 | − |
| myo-3 | Body wall muscles | 0/3 | − |
a Ten L4 larvae of mutant or transgenic animals were transferred to a fresh plate seeded with OP50 to determine whether the animals could proliferate after 1 week.
b Lethality in transgenic pst-1(tm3364) mutant embryos that carried Pdpy-7::pst-1b::egfp was higher than in those that carried Punc-119::pst-1b::egfp or pst-1bc(fl)::egfp.
c Two independent transgenic lines were tested.