FIGURE 4.

NOX1-targeted knockdown mimics EGFR knockdown effects. FaO cells were transfected with either an unsilencing siRNA (uns siRNA), a specific siRNA for NOX1 (NOX1 siRNA), or a specific siRNA for EGFR (EGFR siRNA). A, EGFR and NOX1 expression by real time PCR at 12 h. B, cell number at different times. C, DNA synthesis at 48 h. D, Western blot at 12 h (a representative experiment), indicating the mean of the densitometry analysis of three Western blots for each protein respective to β-actin, used as loading control. All data were calculated relative to cells transfected with unsilencing siRNA. Data represent the mean ± S.E. of three independent experiments. Student's t test was calculated as unsilencing siRNA versus specific siRNA. *, p < 0.05; **, p < 0.01; ***, p < 0.001.