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. 2010 Jun 7;285(32):24855–24862. doi: 10.1074/jbc.M110.118612

FIGURE 2.

FIGURE 2.

The residues of DBL3x important for binding CSA. Wild-type DBL3x (red peak) binds to CSA-expressing CHO K1 cells in a flow cytometry assay. DBL3x proteins bearing the subdomain 3 mutations R1467A and R1503A (blue and green peaks) show significant reductions in binding to CSA on CHO K1 cells. In contrast, the Subdomain 2 double mutation K1324A,K1327A (orange peak) has little effect, binding CSA almost like wild-type DBL3x (red peak). Anti-DBL3x antibody alone (black peak) is shown as a negative control.