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Subdomain 3 of DBL3x alone can bind CSA. S3 binds to CSA expressed on the surface of CHO-K1 cells (magenta peak). Preincubating S3 with bovine tracheal CSA (S3 + CSA, cyan peak) considerably reduces S3 binding to the cells. Anti-DBL3x antibody alone (black peak) is shown as a negative control.
